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How to Simplify ADC Characterization
Characterizing a lysine-conjugated ADC with 3-4 drug molecules scattered across dozens of possible sites is a daunting analytical challenge — but with the right enzymatic strategy and analytical workflows, it becomes straightforward. Arginine-specific digestion with GingisREX eliminates the missed cleavages that plague trypsin-based peptide mapping of conjugated lysines, producing cleaner chromatograms and more reliable quantification. When paired with Byos intact reconstruction, peptide-level and intact-level results can be directly compared — giving you orthogonal confirmation of drug loading in a single analysis.
In this webinar, Genovis will introduce their GlyCLICK® platform for site-specific ADC generation and their enzymatic tools for ADC characterization, followed by Protein Metrics demonstrating how Byos workflows — PTM analysis, ADC intact DAR calculation, and intact reconstruction — enable comprehensive characterization of lysine-conjugated ADCs.
What You Will Learn
- How site-specific conjugation with GlyCLICK simplifies ADC generation and analysis
- Why arginine-specific digestion with GingisREX produces cleaner peptide maps for lysine-conjugated ADCs
- How to use Byos PTM and ADC workflows for peptide-level and intact-level characterization
- How intact reconstruction correlates peptide mapping data with intact mass data for orthogonal DAR confirmation
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